›› 2011, Vol. 23 ›› Issue (2): 114-118.doi: 10.3969/j.issn.1004-616x.2011.02.008

• 论著 • 上一篇    下一篇

甲状腺乳头状癌组织中DAPK1与RARβ的表达及临床意义

武 爽1，古丽扎热叶•艾库拉2，纳菲沙•卡德尔1，阿布力孜•阿布杜拉2，夏米西努尔•伊力克1,﹡   

  1. 1. 新疆医科大学基础医学院细胞生物教研室；2. 新疆医科大学基础医学院生化教研室，新疆 乌鲁木齐 830011)
  • 收稿日期:2010-11-11 修回日期:2010-12-10 出版日期:2011-03-30 发布日期:2011-03-30
  • 通讯作者: 武 爽

Expression and clinopathological significances of DAPK1 and RARβ in papillary thyroid carcinoma tissues in Xinjiang

WU Shuang1, Gulizhareye •Aikula2, Nafeisha•Kadeer1， Abulizi •Abudula2，Xiamixinuer·Yilike1,*   

  1. 1. Biology Department of Pre-clinical College of Xinjiang Medical University; 2. Biochemistry Department of Pre-clinical College of Xinjiang Medical University，Urumqi 830011, Xinjiang, China)
  • Received:2010-11-11 Revised:2010-12-10 Online:2011-03-30 Published:2011-03-30
  • Contact: WU Shuang

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摘要： 通过检测新疆地区甲状腺乳头状癌组织及甲状腺良性病变组织中DAPK1和RARβ的蛋白表达特点，探讨其在甲状腺乳头状癌发生发展中的意义。 方法： 采用免疫组织化学S-P法，检测DAPK1与RARβ在新疆地区38例甲状腺乳头癌、21例甲状腺良性腺瘤、13例桥本氏甲状腺炎、6例结节性甲状腺肿和6例癌旁正常甲状腺组织中的蛋白表达，并分析其与临床病理因素间的关系。 结果： DAPK1在甲状腺乳头状癌中的阳性表达明显低于甲状腺腺瘤、桥本氏甲状腺炎、结节性甲状腺肿和癌旁正常甲状腺组织(P＜0.01)；RARβ在甲状腺乳头状癌中的阳性表达明显高于在甲状腺良性腺瘤、桥本氏甲状腺炎、结节性甲状腺肿和癌旁正常甲状腺组织(P＜0.01)；DAPK1与RARβ在甲状腺乳头状癌中的表达与患者的年龄、性别、民族无明显相关关系(P＞0.05)。 结论： DAPK1可能参与了甲状腺乳头状癌的发生发展过程。检测DAPK1和RARβ的表达水平可作为判断甲状腺乳头状癌转移和预后的参考指标。

关键词: DAPK1, RARβ, 甲状腺癌, 免疫组织化学, 表达

Abstract: To investigate the expressions of death-associated protein kinase-1(DAPK1) and retinoic acid receptor beta (RARβ) in different thyroid carcinoma tisssues and clinicopathological significance in thyroid cancer． METHODS: S-P immunity histochemistry was used to detect DAPK1,RARβ in 38 thyroid carcinomas (PTC), 21 thyroid adenomas，13 Hashimoto’s thyroiditis , 6 nodular goiters and 6 peri-tumor tissue. RESULTS: The positive rate of DAPK1 in PTC was significantly lower than those in thyroid adenoma, Hashimoto’s thyroiditis, nodular goiters and peri-tumor tissue(P<0.01). The positive rate of RARβ in PTC was significantly higher than those in thyroid adenoma, Hashimoto’s thyroiditis, nodular goiters and peri-tumor tissue (P<0.01). CONCLUSION: As an apoptotic agent, DAPK1 may function as an inhibitor of tumor, and low DAPK1 gene expression may be involved in the oncogenesis of PTC. The detection of DAPK1 expression may be helpful for assessing metastasis and prognosis of PTC. RARβ expression was significantly higher in PTCs than that in benign thyroid lesions，and this characteristics can be of important value in diagnosis.

Key words: death-associated protein kinase-1, retinoic acid receptor beta, thyroid carcinoma, immunity histochemistry, expression

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